Thursday 23 May 2013
   
 

Kinetic study and characterization of 1,4--endoglucanase of Aspergillus niger ANL301

Kinetic study and characterization of 1,4--endoglucanase of Aspergillus niger ANL301

Submerged fermentation of Aspergillus niger ANL 301, in basal medium containing cellulose as sole carbon source, yielded crude extracellular proteins with 0.54 ± 0.02 units mg protein-1 of 1, 4- β-endoglucanase activity. Partial purification by ammonium sulphate precipitation (80% saturation) and gel filtration on Sephadex 25-300 gave two active fractions of 1, 4- β-endoglucanase, which...
 
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Shalom Nwodo Chinedu, O.C. Nwinyi, U.A. Okafor and V.I. Okochi
Shalom N. Chinedu, Ph.D. Department of Biological Sciences (Biochemistry & Molecular Biology) School of Natural & Applied Sciences College of Science & Technology Covenant University view full profile
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